Extraction of Isovitexin from Melinjo (Gnetum Gnemon L.) Leaves Using Mixtures of Liquid Carbon Dioxide and Ethanol

Yusraini Dian Inayati Siregar; Kenji Mishima; Ryo Kawakami; Shota Ito; Tetsuya Hirota; Tanjina Sharmin; Takafumi Kato; Takunori Harada; Makoto Misumi; Hideaki Orii; Tadashi Suetsugu; Keiichi Irie; Kenichi Mishima; Kumiko Sakai; Kenji Sakai; Hirofumi Kawamura; Hilyatuz Zahroh; Nurelela; Adi Riyadahi; Lily Surayya Eka Putri; Agus Salim

Authors

Yusraini Dian Inayati Siregar Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, 8-19-1, Nanakuma Jonan-ku, Fukuoka 814-0180, Japan Author
Kenji Mishima Academia, Industry, Government Collaborative Research Institute of Composite Material, Fukuoka University, 8-19-1, Nanakuma Jonan-ku, Fukuoka 814-0180, Japan Author
Ryo Kawakami Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, 8-19-1, Nanakuma Jonan-ku, Fukuoka 814-0180, Japan Author
Shota Ito Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, 8-19-1, Nanakuma Jonan-ku, Fukuoka 814-0180, Japan Author
Tetsuya Hirota Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, 8-19-1, Nanakuma Jonan-ku, Fukuoka 814-0180, Japan Author
Tanjina Sharmin Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, 8-19-1, Nanakuma Jonan-ku, Fukuoka 814-0180, Japan Author
Takafumi Kato Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, 8-19-1, Nanakuma Jonan-ku, Fukuoka 814-0180, Japan Author
Takunori Harada Department of Applied Chemistry, Faculty of Engineering, Oita University, 700, Dannoharu, Oita, Oita 870-1124, Japan Author
Makoto Misumi Department of Electronics Engineering and Computer Science, Faculty of Engineering, Fukuoka University, 8-19-1, Nanakuma Jonan-ku, Fukuoka 814-0180, Japan 5Department of Neuropharm Author
Hideaki Orii Academia, Industry, Government Collaborative Research Institute of Composite Material, Fukuoka University, 8-19-1, Nanakuma Jonan-ku, Fukuoka 814-0180, Japan Author
Tadashi Suetsugu Department of Electronics Engineering and Computer Science, Faculty of Engineering, Fukuoka University, 8-19-1, Nanakuma Jonan-ku, Fukuoka 814-0180, Japan Author
Keiichi Irie Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1, Nanakuma Jonanku, Fukuoka 814-0180, Japan Author
Kenichi Mishima Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1, Nanakuma Jonanku, Fukuoka 814-0180, Japan Author
Kumiko Sakai Research Promotion Institute, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu-Shi, Oita, 879-5593, Japan Author
Kenji Sakai Department of Bioscience and Biotechnology, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan Author
Hirofumi Kawamura Department of Seasoning & Foods Division, San-Ei Gen F.F.I., Inc., 1-1-11, Sanwa-cho, Toyonaka, Osaka 561-8588, Japan Author
Hilyatuz Zahroh Faculty of Science and Technology, Syarif Hidayatullah State Islamic University (UIN) Jakarta, JL.Ir.H.Juanda Ciputat, Tangerang, 15412, Indonesia Author
Nurelela Faculty of Science and Technology, Syarif Hidayatullah State Islamic University (UIN) Jakarta, JL.Ir.H.Juanda Ciputat, Tangerang, 15412, Indonesia Author
Adi Riyadahi Faculty of Science and Technology, Syarif Hidayatullah State Islamic University (UIN) Jakarta, JL.Ir.H.Juanda Ciputat, Tangerang, 15412, Indonesia Author
Lily Surayya Eka Putri Faculty of Science and Technology, Syarif Hidayatullah State Islamic University (UIN) Jakarta, JL.Ir.H.Juanda Ciputat, Tangerang, 15412, Indonesia Author
Agus Salim Faculty of Science and Technology, Syarif Hidayatullah State Islamic University (UIN) Jakarta, JL.Ir.H.Juanda Ciputat, Tangerang, 15412, Indonesia Author

Keywords:

Isovitexin

Abstract

Isovitexin from Melinjo (Gnetum gnemon L.) leaves was extracted using mixtures of liquid CO2 and ethanol. For finding out the optimum extraction condition, extraction tests were performed at 5, 20 and 25 °C under pressures ranging from 8 to 14 MPa. A conventional extraction by ethanol was performed to compare the extraction yield of isovitexin. We conducted qualitative and quantitative analyses for isovitexin in the extract by using HPLC. The effects of three operating parameters, including temperature, pressure and the mole fraction of ethanol in liquid CO2, on the extraction yield were investigated using the single-factor method. The yield of isovitexin in the extract was significantly improved by adjusting the extraction temperature and the solvent composition.